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Strand Displacement Amplification: A Powerful Tool for Rapid DNA Amplification
Strand displacement amplification (SDA) is an isothermal DNA amplification technique that enables rapid and sensitive detection of nucleic acids without thermal cycling.
It uses enzymes that synthesize new DNA strands while simultaneously displacing existing strands, enabling continuous amplification.
SDA is used in infectious disease detection, genetic testing, molecular diagnostics, and point-of-care applications. Because it does not require a thermal cycler, SDA is faster and more portable than PCR. The method relies on nicking enzymes and polymerases that work at a constant temperature, generating amplified products within minutes.
Its high sensitivity makes it useful for detecting low levels of pathogens or genetic variations. Combined with microfluidics and portable diagnostic platforms, SDA supports rapid testing in remote areas, emergency care, and field laboratories.